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Percutaneous Epididymal Sperm Aspiration (PESA)

For the zero sperm count (azoospermia) man, sperm can be retrieved in some cases from the portion above the testis called the epididymis for example in cases of congenital absence of the vas deferens - a condition in which both the tubes transporting sperm to the exterior are absent from birth. The sperm thus retrieved by our expert andrologists, Dr Rupin Shah or Dr VijayKulkarni can then be used for injecting the wife's oocytes by ICSI and thereby help give the couple their own biological offspring.

Testicular Sperm Aspiration (TESA)

For some patients with a zero sperm count (azoospermia), sperm can often be retrieved directly from the testes using a fine needle aspiration technique - immature sperm thus retrieved can then be matured in vitro and injected into the wife's oocytes by ICSI to achieve a viable pregnancy and live birth.

Micro Tese

This is the latest technique incorporated by our team for cases of azoospermia where sperms are not retrieved by conventional PESA or TESA. Here, under an operating microscope, healthy seminiferous tubules are extracted from the testes and then dissected to find sperms.

Steps Involved in ICSI

The mature egg is held with a specialized holding pipette.

A very delicate, sharp and hollow needle is used to immobilize and pick up a single sperm (which has been retrieved from the epididymis or testicle by PESA or TESA respectively).

This needle is then carefully inserted through the zona (shell of egg) and in to the cytoplasm of the egg.

The sperm is injected in to the cytoplasm and the needle carefully removed. The eggs are checked the next morning for evidence of normal fertilization.

Embryo transfer is then done.

(please see section on IVF & ICSI for more details)

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